֪RcY(ji)
Y(ji)ǰһrεČW(xu)(x)rM(jn)һȫϵy(tng)ĿY(ji)ʹ҂Чʣ҂һW(xu)(x)Y(ji)ô֪Y(ji)ΌСʹij֪RcY(ji)gӭxϣ܉ϲg
֪RcY(ji) 1
(yng)(wĴ̼a(chn)һ(yng)(yng)DŽӑB(ti)^)cm(yng)((yng)ԣҲoB(ti)m(yng)ֲıo(h)ɫ)ɻzQ
﹤̰֣քeǻ:(x)(μg(sh))l(f)ø̡
Ĺͬ|(zh)A(ch)(Ԫ)NNRNADNAнY(ji)(gu)ʽY(ji)(gu)(Ǿa^(q);a^(q))zܴaȡ
Ԫغռ(x)rĶٵքeOCHNPS(x)ԪC
wПoC(j)}ĹܺãȱF(do)¼t(x)\(yn)ݔ½wF(xin)Sּ(x);ȱF(do)ؚѪwF(xin)SwΘ(gu)ɏ(f)s
ֲ:(x)е|(zh)քeǣ֬|(zh);:(x)еҪ|(zh)Ҫ֬͵|(zh)^(q)ֱԴҪԴԴԴ
|(zh)Y(ji)(gu)ԭ(4)DNAY(ji)(gu)ԭ(3)DNAY(ji)(gu)(wn)ԭ(3)
(x)СˮƽR¿ɿֱС0.2ļ(x)Y(ji)(gu)Сļ(x)֧ԭw
|(zh)ĻԪCHONSԪ;ĻԪCHONPPԪ;ѪtԪCHONFe~GصԪCHONMgԪCHON;V|(zh)Ԫص֬
ԭ˼(x)cТٟoĤʢڟoȾɫwۃHкwܼ(x)ڳɷľǢzѭҎ(gu)ɢރHеĿz׃ǻͻ׃ߟoĤϵy(tng)Y(ji)(gu)a^(q)Bm(x);t(x)o(x)˺;wѣM(jn)Пoȡ(x)Ĥĺò
(ni)|(zh)W(wng)Ĥϵy(tng)ģc(x)ĤB(ni)cӺĤB߀cwĤB|(zh)M(jn)ۯBMbǻȼӹγɾĤС\(yn)ݔ?sh)ߠwM(jn)һӹͷ
ڵпwɔ_(ǵ)øԭȍuL(jng)^ĤԼ(x)Y(ji)(gu)Ѓ(ni)|(zh)W(wng)ߠwͼ(x)Ĥ
N(x)к˻Ҫȏ(f)ƽ|(zh)SC(j)ȷֻŷֳɼ(x)zͼ(x)|(zh)zɷNʽ(x)vδֻֻ˥ϡѴΔ(sh)Խļ(x)ԽL(x)˥̓(ni)ͬõĽY(ji)
(x)ֻČ|(zh)ǻxԱ_(d)D(zhun)ˮƽɻɂ(c)Ǿa^(q){(dio)ص
(x)ȫָѷֻĵļ(x)аl(f)ĝ(j):(x)ȫԴСɷ֞ȫԼ(x)(̥(x))(ԭc(x))(Ѫɼ(x));(j)ֲ:(x)_(d)ȫԴСǣֳܾ(x)w(x);ȫԵ|(zh)A(ch)Ǽ(x)(ni)бNȫz|(zh)
Ӱøٷ(yng)ٶȵøȡضAȵʹø׃ԵǏ(qing)ᡢ(qing)Aߜw(ni)øĻԲضӰ푡-ømض60
̵Ĺøԃ(ni)øDNABø(cI);(x)̵ĹøwSøzø(@ԭ|(zh)wrm˝ȵҺCȝBo(h)ԭ|(zh)w)ȵø(:(x))
ATPǼ(x)(ni)ֱԴ|(zh)ڼ(x)(ni)cADPD(zhun)ܵ\(yn)ݔźͷ(yng)տs(jng)(do)ЙC(j)ϳɵ;ܵНBv;γATPǺúⷴ(yng)
D(zhun)׃ɻSW(xu)ܕrӹwˮKwoø(NADP+)هB(ti)~GaӡˮNADPHγɵăɂʽ
߹ʵķ1LÕrg(һ(ni)݆)2ӹe(ֲg)3߹Ч(ٶ)
B܄(ˮͪƾ)ͨ^ĤĔU(ku)ɢȑ(yng)QĦȣǰٷ֝
vˮ\(yn)ݔˮֵĄҲ\(yn)ݔxӵĄ;ֲˮĄ߀Ǹ;Ӱvõǜضȡ(ض)L(fng)ֲˮ(1%--5%)vȴrF(xin)ˮֲLĘ(bio)־
Ҫ(j)ֲͬͬˮͬ(ji)M(jn)ɲÇιM(jn)M(jn)й(ji)sˮ
oֳ(Ă)¡g(sh)ֲMB(yng)ֲָ̥g(sh)(yu)cDZHă(yu)ԠֲMB(yng)ă(yu)cǣȡB(yng)ڶֳʸԄӻ(yng)Уٷֳ;oֲ;a(chn)ˎʳƷӄɫx;˹N;D(zhun)ֲ
ֲˮֺ͌xӵǃɂ(d)^עДٶȴС
w(ni)|(zh)āԴҪʳ֬ԴҪǸߵD(zhun)
|(zh)w(ni)܃Ǽ(x)ĽY(ji)(gu)|(zh)|(zh)Դ|(zh)Óֽܷܡ|(zh)Ól(f)ڣ|(zh)z^ࡢոz뵰|(zh)|(zh)ֽ^I
wÿ횔zһĵ|(zh)ԭǵ|(zh)Ǽ(x)ĽY(ji)(gu)|(zh)|(zh);|(zh)w(ni)܃;D(zhun)òγзNİ;|(zh)w(ni)ÿ춼¡(횰ɫهKi)
^(q)eȾɫwM(һͬԴȾɫw)ȾɫwM(w(ni)ȾɫwN͔(sh)Ŀ)ȾɫwM(磺Ѽ(x)ȾɫwMɣ22l+X);Ի(ˮ24)ĆαM12lȾɫwzϢ;˻M(wDNAyȫzϢ)ˆαM(24lȾɫwzϢ);
ЙC(j)xP(gun)ϵ((lin)ϵƼs)D(zhun)(֬ܵ|(zh));D(zhun)Зl(ǹ(yng)D(zhun)׃?yu)֬ǿɴD(zhun)׃?yu)ֻ֬֬D(zhun)׃?yu)?;Ƽs(ֻǴxϵKr֬|(zh));ǴxĘм~
֪RcY(ji) 2
1.(x)W(xu)fĽ^
(1)(x)W(xu)fĄ(chung)ʼʩRǺʩ
(2)(x)W(xu)fҪcǣ(x)һЙC(j)wһЄֲﶼɼ(x)l(f)ɼ(x)ͼ(x)a(chn)(gu);(x)һ(d)ĆλԼc(x)ͬMɵw;¼(x)ɏϼ(x)Юa(chn)
(3)(x)W(xu)fĄ(chung)M(jn)Ҫxǣʾκ΄ֲɼ(x)(gu)ɵģĶfֲ֮gһHP(gun)ϵ֮gHP(gun)ϵʾM(jn)Ҫrֵ
2.Nӵļ(x)
(4)Ȼϵy(tng)ČӴУ(x)Mϵy(tng)wNȺȺB(ti)ϵy(tng)Ȧ
(5)ֲϵy(tng)ӴЛ]Сϵy(tng)@Ӵ
(6)ԭ˼(x)c˼(x)ı|(zh)^(q)eПoԺĤļ(x)
չ
ԭ˼(x)wo(x)ԭ缚(x)ļ(x)Ҫɷc|(zh)Y(ji)϶ɵĻ
ԭzϵ zҎ(gu);ֳ^˻zϵ zҎ(gu)
ȻlԭĿz׃ֻлͻ׃;Ŀz׃лͻ׃ؽMȾɫw׃
ԭ˼(x)缚(x)ҪԶѵķʽM(jn)з;˼(x)ķѷʽнzozp(sh)
(7)ܪ(d)Ĵxͷֳ^ֻĻ(x)M(jn)
չ
ٲǼȲԭҲ
ڽMÿNĻλķNÓķNǺᡣ
۲B(yng)ֱB(yng)B(yng)鲡ķֳĻ(x)M(jn)
3.(x)Ĥϵy(tng)ĽY(ji)(gu)
(8)ò.t(x)ܷxõļ(x)ĤѼ(x)ˮˮM(jn)뼚(x)Ѽ(x)qƣ(x)(ni)|(zh)@ӾͿԵõļ(x)Ĥ
(9)(x)ĤҪ֬|(zh)͵|(zh)M߀
չ
ʹ(x)Ĥ|(zh)M(jn)ܵ|(zh)dw
ڼ(x)ĤcĤĻW(xu)MɴͬڲͬĤW(xu)|(zh)ĺвe磬(x)Ĥĺc(x)ĤҪࡣ
(10)(x)ĤĽY(ji)(gu)cx^ԡ
(11)ڼ(x)Ĥһɼ(x)Ĥϵĵ|(zh)cY(ji)϶ɵǵףDZDZc(x)ReP(gun)ϵͺƤ(x)ǵбo(h)͝
(12)ֲ:(x)ڵĻW(xu)ɷҪwSغz
չ
ټ(x)(x)ڵijɷc|(zh)Y(ji)϶ɵĻ
ڳwSøzøȥֲ:(x)
֪RcY(ji) 3
1zy߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMc߀ԭǰl(f)(yng)ɴutɫʹÕrעF(xin)F(xin)
2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ
3bMеĵ|(zh)psԇʹÕrȼNaOHҺ2~3CuSO4Һ(yng)ɫj(lu)
4oȾɫwȾɫõȾɫđϻtҺ
5^DNARNAڼ(x)еķֲüG_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ
6ԭ|(zh)Ӱ(x)ĤҺĤԼ@ɌĤ֮gļ(x)|(zh)
7ǾGȾҺnjһȾwĻ(x)Ⱦʹ(x)оwʬF(xin){(ln)Gɫ
8ڷڵĺϳӹ\(yn)ݔͷ^P(gun)ļ(x)w(ni)|(zh)W(wng)ߠww
9γҪͨ^Óˮsϵķʽ
10(dng)Һȴڼ(x)Һȕrֲ:(x)l(f)|(zh)ڷxF(xin);(dng)ҺСڼ(x)Һȕrֲ:(x)l(f)|(zh)ڷxď(f)ԭF(xin)
֪RcY(ji) 4
һ W(xu)гҊW(xu)Ԫؼã
1Cawȱ֮ܛѪҺCa2+͕鴤^߄toѪҺеCa2+дM(jn)ѪҺ̵ãÙccѪҺеCa2+ѪҺͲl(f)ֲвٵԪһȱ۵ĽMܵ
2FeѪtĽMɳɷȱȱFؚѪѪtеFeǶrFrFDzõֲвٵԪһȱ۵ĽMܵ
3Mg~GwĽMԪءܶøļֲȱVr~׳F(xin)~}ʧG
4BM(jn)۵Ȱl(f)ͻ۹ܵLȱֲF(xin)
5Iנټصijɷȱ׃СY˕طԼנ[
6KѪ⛺^͕rF(xin)ļԄӹ(ji)ɮ(do)ʧ
7NNǘ(gu)~GATP|(zh)ͺıԪNֲw(ni)γɵĻﶼDz(wn)ĻˮNֲw(ni)ƄӣȱNr~~N(do)~SNһNˮB(ti)ϵy(tng)IB(yng)һNW(xu)ԪˮB(ti)ϵy(tng)У^NcPϕɸIB(yng)ڵˮB(ti)ϵy(tng)еĸIB(yng)Q顰ˮAںB(ti)ϵy(tng)еĸIB(yng)Q顰ೱw(ni)ȱNHȱٰ͕Ӱ푵wLl(f)
8PPǘ(gu)֬ATPıԪءֲw(ni)ȱPӰ푵DNAď(f)ƺRNAD(zhun)ĶӰ푵ֲLl(f)P߀cֲúͺеf^ATPADPжPҲˮB(ti)ϵy(tng)IB(yng)һNԪֲȱPr~׳F(xin)o~Gϼtɫt
9ZnijЩøĽMɳɷҲøĻġͽzϳɫøкZn]ZnͲܺϳᡣȱZnOҵֲС~Yͅ~Y~׃С(ji)gs
W(xu)гõԇ
1ԇ ɷ֣0.1g/ml NaOHҺ0.05g/ml CuSO4Һ÷ԇҺҺweٌϺԇyҺˮԡӟֱӼӟyҺд߀ԭǣtʴutɫ
2Ƕԇ{(ln)ɫҺǻϺˮԡF(xin)utɫǵĜy
3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷yҺȼ2mlҺuм3~4Һuyдڵ|(zh)tʬF(xin)ɫ
4K÷ȡKw95%ľƾuڙzy֬Ɍ֬ȾSɫKȾɼtɫ
5bDNADNAˮȾ{(ln)ɫ
6GbDNADNAGأȾ{(ln)Gɫ
750%ľƾҺ֬bУKȾҺȾɫ50%ľƾҺϴȥɫ
875%ľƾҺښ75%ľƾܝB뼚(x)(ni)ʹ|(zh)׃@ƾĝBÓˮÜp(qing)@ȣtʹ(x)浰|(zh)ѸÓˮ̳ĤKƾ룬75ľƾҺg(sh)ǰQˎᘾǰƤwÓԼC(j)е
995%ľƾҺswe?jn)?sh)95%ľƾDNA
1015%}95%ľƾҺweϿڽx
11đҺ(Ȟ0.01g/ml0.02g/ml)ȾɫwɫɌȾɫwȾɫͨȾɫ3~5ҲôtȾɫ
1220%ĸK3%^3.5%ȻFڱ^^øFe3+ĴЧrĸKк^ø
133%ĿԵҺ3%Һ2%røҺ̽øۺǵÌ
14Һb۵Ĵ׃{(ln)
15ͪȡ~Gwеɫ
16Һɷ֣20ʯ2ݱͪ1ݱ϶ɣҲ93̖ͣɫصČɫڞVϷx_
17裺ɫصȡķxĥGɫ~Ƭrʹĥ
18̼}ĥGɫ~ƬrкЙC(j)ֹĥr~GwеɫƉ
190.3g/mLҺஔ(dng)30%Һֲ:(x)Һĝȴ|(zh)ڷx
200.1g/mLęcҺcuѪѪ
21ȻcҺٿܽDNA(dng)ȻcȞ2mol/L 0.015mol/LrDNAܽߣȻcȞ0.14 mol/LrDNAܽߡڝȞ0.9%r}ˮ
22ȵøٿÁֽ⵰|(zh)ڿڄ:(x)B(yng)rֽMʹM(x)ɢ
23ˮأ˹T(do)wԇȰl(f)ķNӻ磬ʹȾɫwMӱԭǿڷѵļ(x)Nwγɡ
24Ȼ}Ӽ(x)(x)ڵͨԣڻ̵D(zhun)ʹ(x)̎ڸܑB(ti)
֪RcY(ji)СP(gun)£
αһ֪RcY(ji)02-19
Y(ji)03-16
tlj֪RcY(ji)12-12
tlj֪RcY(ji)12-11
γ֪RcY(ji)ͨ13ƪ08-30
Z֪Rc֮ZR06-20
֪Rc֪Rc07-28
ˮGij֪Rc10-19
tlj֪Rc12-20