֪R(sh)c(din)Y(ji)
Y(ji)ǰһ(g)r(sh)εČW(xu)(x)rM(jn)һȫϵy(tng)ĿY(ji)ʹ҂Ч҂һW(xu)(x)Y(ji)ô֪Y(ji)ΌСʹij֪R(sh)c(din)Y(ji)gӭxϣ܉ϲg
֪R(sh)c(din)Y(ji) 1
(yng)((g)w(du)Ĵ̼(hu)a(chn)һ(yng)(yng)DŽ(dng)B(ti)^)cm(yng)((yng)ҲoB(ti)m(yng)(dng)ֲıo(h)ɫ)ɻzQ
﹤̰֣քeǻ:(x)(μg(sh))l(f)ø
Ĺͬ|(zh)A(ch)(Ԫ)NNRNADNAнY(ji)(gu)ʽY(ji)(gu)(Ǿa^(q);a^(q))zܴa
Ԫغռ(x)rĶٵքeOCHNPS(x)ԪC
wПoC(j)}ĹܺãȱF(do)¼t(x)\(yn)ݔ½wF(xin)Sּ(x)(dng);ȱF(do)ؚѪwF(xin)Sw(dng)áΘ(gu)ɏ(f)s
ֲ:(x)е(ch)|(zh)քeǣ֬ۡ|(zh);(dng):(x)еҪ(ch)|(zh)Ҫ֬͵|(zh)^(q)ֱԴҪԴ(ch)ԴԴ
|(zh)Y(ji)(gu)ԭ(4(g))DNAY(ji)(gu)ԭ(3(g))DNAY(ji)(gu)(wn)ԭ(3(g))
(x)СˮƽR¿ɿֱС0.2ļ(x)Y(ji)(gu)Сļ(x)֧ԭw
|(zh)ĻԪCHONSԪ;ĻԪCHONPPԪ;ѪtԪCHONFe~GصԪCHONMgԪCHON;V|(zh)Ԫص֬
ԭ˼(x)c(din)ТٟoĤʢڟoȾɫwۃHкwܼ(x)ڳɷľǢzѭҎ(gu)ɢރHеĿz׃ǻͻ׃ߟoĤϵy(tng)Y(ji)(gu)a^(q)Bm(x);(dng)t(x)o(x)˺;wM(jn)Пoȡ(x)Ĥĺò
(ni)|(zh)W(wng)Ĥϵy(tng)ģc(x)ĤB(ni)cӺĤB߀cwĤB(du)|(zh)M(jn)ۯBMbǻȼӹγɾĤС\(yn)ݔ?sh)ߠwM(jn)һӹͷ
ڵпwɔ_(ǵ)øԭȍuءL(jng)^ĤԼ(x)Y(ji)(gu)Ѓ(ni)|(zh)W(wng)ߠwͼ(x)Ĥ
N(x)к˻Ҫȏ(f)ƽ֣|(zh)SC(j)ȷֻŷֳɼ(x)zͼ(x)|(zh)zɷNʽ(x)vδֻֻ˥ѴΔ(sh)Խļ(x)ԽL(x)˥̓(ni)ͬõĽY(ji)
(x)ֻČ(sh)|(zh)ǻxԱ_(d)D(zhun)ˮƽɻɂ(c)Ǿa^(q){(dio)ص
(x)ȫָѷֻĵļ(x)аl(f)ĝ(j)(dng):(x)ȫԴСɷ֞ȫԼ(x)((dng)̥(x))(ԭc(x))(Ѫɼ(x));(j)ֲ:(x)_(d)ȫԴСǣֳܾ(x)w(x);ȫԵ|(zh)A(ch)Ǽ(x)(ni)бNȫz|(zh)
Ӱøٷ(yng)ٶȵøضȡAȵʹø׃ԵǏ(qing)ᡢ(qing)Aߜء(dng)w(ni)øĻԲضӰ-ømض60
̵Ĺøԃ(ni)øDNABø(cI);(x)̵ĹøwSøzø(@ԭ|(zh)wr(sh)m˝ȵҺCȝBo(h)ԭ|(zh)w)ȵø((dng):(x))
ATPǼ(x)(ni)ֱԴ|(zh)ڼ(x)(ni)cADPD(zhun)ܵ(dng)(dng)\(yn)ݔźͷڡ(yng)տs(jng)(do)늡ЙC(j)ϳɵ;ܵНBv;γATP(dng)Ǻúⷴ(yng)
D(zhun)׃ɻSW(xu)ܕr(sh)ӹwˮKwoø(NADP+)هB(ti)~GaӡˮNADPHγɵăɂ(g)ʽ
߹ʵķ1LÕr(sh)g(һ(ni)݆)2ӹe(ֲg)3߹Ч(ٶ)
B܄(ˮͪƾ)ͨ^ĤĔU(ku)ɢȑ(yng)QĦȣǰٷ֝ȡ
vˮ\(yn)ݔˮֵĄ(dng)Ҳ\(yn)ݔxӵĄ(dng);ֲˮĄ(dng)߀Ǹ;Ӱvõǜض(ض)L(fng)ֲˮ(1%--5%)vȴr(sh)F(xin)ˮֲLĘ(bio)־
Ҫ(j)ֲͬͬˮͬ(ji)M(jn)ɲÇࡢιM(jn)M(jn)й(ji)sˮ
oֳ(Ă(g))(dng)¡g(sh)ֲMB(yng)(dng)ֲָ̥g(sh)(yu)c(din)DZHă(yu)ԠֲMB(yng)ă(yu)c(din)ǣȡ١B(yng)ڶֳʸߡԄ(dng)(yng)Уٷֳ;oֲ;a(chn)ˎʳƷӄɫx;˹N;D(zhun)ֲ
ֲ(du)ˮֺ͌(du)xӵǃɂ(g)(du)(d)^עДٶȴС
w(ni)|(zh)āԴҪʳ֬ԴҪǸߵD(zhun)
|(zh)w(ni)܃(ch)Ǽ(x)ĽY(ji)(gu)|(zh)|(zh)Դ|(zh)Óֽܷ|(zh)Ól(f)ڣ|(zh)z^ոz뵰|(zh)|(zh)ֽ^I
wÿ횔zһĵ|(zh)ԭǵ|(zh)Ǽ(x)ĽY(ji)(gu)|(zh)|(zh);|(zh)w(ni)܃(ch);D(zhun)òγзNİ;|(zh)w(ni)ÿ춼(횰ɫهKi)
^(q)eȾɫwM(һͬԴȾɫw)ȾɫwM((g)w(ni)ȾɫwN͔(sh)Ŀ)ȾɫwM(磺Ѽ(x)ȾɫwMɣ22l+X);Ի(ˮ24)ĆαM12lȾɫwzϢ;˻M(wDNAyȫzϢ)ˆαM(24lȾɫwzϢ);
ЙC(j)xP(gun)ϵ((lin)ϵƼs)D(zhun)(֬ܵ|(zh));D(zhun)Зl(ǹ(yng)D(zhun)׃?yu)֬ǿɴD(zhun)׃?yu)ֻ֬֬D(zhun)׃?yu)?;Ƽs(ֻǴxϵKr(sh)֬|(zh));ǴxĘм~
֪R(sh)c(din)Y(ji) 2
1.(x)W(xu)fĽ^
(1)(x)W(xu)fĄ(chung)ʼʩRǺʩ
(2)(x)W(xu)fҪc(din)ǣ(x)һ(g)ЙC(j)wһЄ(dng)ֲﶼɼ(x)l(f)ɼ(x)ͼ(x)a(chn)(gu);(x)һ(g)(du)(d)ĆλԼ(du)c(x)ͬMɵw;¼(x)ɏϼ(x)Юa(chn)
(3)(x)W(xu)fĄ(chung)(du)M(jn)Ҫxǣʾκ΄(dng)ֲɼ(x)(gu)ɵģĶf(dng)ֲ֮gһHP(gun)ϵ֮gHP(gun)ϵ(du)ʾM(jn)Ҫr(ji)ֵ
2.Nӵļ(x)
(4)Ȼϵy(tng)ČӴУ(x)Mϵy(tng)(g)wNȺȺB(ti)ϵy(tng)Ȧ
(5)ֲϵy(tng)ӴЛ]Сϵy(tng)@(g)Ӵ
(6)ԭ˼(x)c˼(x)ı|(zh)^(q)eПoԺĤļ(x)
չ
ԭ˼(x)w⣬o(x)ԭ缚(x)ļ(x)Ҫɷc|(zh)Y(ji)϶ɵĻ
ԭzϵ zҎ(gu);ֳ^˻zϵ zҎ(gu)ɡ
Ȼl£ԭĿz׃ֻлͻ׃;Ŀz׃лͻ׃ؽMȾɫw׃
ԭ˼(x)缚(x)ҪԶѵķʽM(jn)з;˼(x)ķѷʽнzozѡp(sh)
(7)ܪ(d)Ĵxͷֳ^ֻĻ(x)M(jn)
չ
ٲǼȲԭҲ
ڽMÿNĻλķNÓᣬķNǺ
۲B(yng)ֱB(yng)B(yng)?yn)鲡ķֳĻ?x)M(jn)
3.(x)Ĥϵy(tng)ĽY(ji)(gu)
(8)ò(dng).t(x)(sh)(yn)ܷxõļ(x)ĤѼ(x)ˮˮ(hu)M(jn)뼚(x)Ѽ(x)q(x)(ni)|(zh)@ӾͿԵõļ(x)Ĥ
(9)(x)ĤҪ֬|(zh)͵|(zh)M߀
չ
ʹ(x)Ĥ|(zh)M(jn)ܵ|(zh)dw
ڼ(x)ĤcĤĻW(xu)MɴͬڲͬĤW(xu)|(zh)ĺвe(x)Ĥĺ(du)c(x)ĤҪࡣ
(10)(x)ĤĽY(ji)(gu)c(din)(dng)x^
(11)ڼ(x)Ĥһɼ(x)Ĥϵĵ|(zh)cY(ji)϶ɵǵDZDZc(x)R(sh)eP(gun)ϵͺƤ(x)ǵбo(h)͝
(12)ֲ:(x)ڵĻW(xu)ɷҪwSغz
չ
ټ(x)(x)ڵijɷc|(zh)Y(ji)϶ɵĻ
ڳwSøzøȥֲ:(x)
֪R(sh)c(din)Y(ji) 3
1zy(c)߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMc߀ԭǰl(f)(yng)ɴutɫʹÕr(sh)עF(xin)F(xin)
2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ
3bMеĵ|(zh)psԇʹÕr(sh)ȼNaOHҺ2~3CuSO4Һ(yng)ɫj(lu)
4oȾɫwȾɫõȾɫđϻtҺ
5^DNARNAڼ(x)еķֲüG_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ
6ԭ|(zh)Ӱ(x)ĤҺĤԼ@ɌĤ֮gļ(x)|(zh)
7ǾGȾҺnjһȾwĻ(x)Ⱦʹ(x)оwʬF(xin){(ln)Gɫ
8ڷڵĺϳӹ\(yn)ݔͷ^УP(gun)ļ(x)w(ni)|(zh)W(wng)ߠww
9γ朣Ҫͨ^Óˮsϵķʽ
10(dng)Һȴڼ(x)Һȕr(sh)ֲ:(x)l(f)|(zh)ڷxF(xin);(dng)ҺСڼ(x)Һȕr(sh)ֲ:(x)l(f)|(zh)ڷxď(f)ԭF(xin)
֪R(sh)c(din)Y(ji) 4
һ W(xu)гҊW(xu)Ԫؼã
1Cawȱ֮(hu)ܛѪҺCa2+͕(hu)鴤^߄t(hu)oѪҺеCa2+дM(jn)ѪҺ̵ãÙccѪҺеCa2+ѪҺͲ(hu)l(f)ֲ̡вٵԪһȱ۵ĽM(hu)ܵ
2FeѪtĽMɳɷȱ(hu)ȱFؚѪѪtеFeǶr(ji)Fr(ji)FDzõֲвٵԪһȱ۵ĽM(hu)ܵ
3Mg~GwĽMԪءܶøļֲȱVr(sh)~׳F(xin)~}ʧG
4BM(jn)۵Ȱl(f)ͻ۹ܵLȱֲ(hu)F(xin)(sh)
5Iנټصijɷȱ׃(hu)СY˕(hu)طԼנ[
6KѪ⛺^͕r(sh)(hu)F(xin)ļԄ(dng)(ji)ɮ(do)ʧ
7NNǘ(gu)~GATP|(zh)ͺıԪNֲw(ni)γɵĻﶼDz(wn)ĻˮģNֲw(ni)Ƅ(dng)ȱNr(sh)~~N(do)~SNһNˮB(ti)ϵy(tng)IB(yng)һNW(xu)ԪˮB(ti)ϵy(tng)^NcPϕ(hu)ɸIB(yng)ڵˮB(ti)ϵy(tng)еĸIB(yng)Q顰ˮAںB(ti)ϵy(tng)еĸIB(yng)Q顰ೱ(dng)w(ni)ȱN(sh)Hȱٰ͕(hu)Ӱ푵(dng)wLl(f)
8PPǘ(gu)֬ATPıԪֲw(ni)ȱP(hu)Ӱ푵DNAď(f)ƺRNAD(zhun)䛣ĶӰ푵ֲLl(f)P߀cֲúͺеf^̣?yn)ATPADPжPҲˮB(ti)ϵy(tng)IB(yng)һNԪءֲȱPr(sh)~׳F(xin)o~Gϼtɫt
9ZnijЩøĽMɳɷ֣ҲøĻͽzϳɫøкZn]ZnͲܺϳȱZnOҵֲС~Yͅ~Y~׃С(ji)gs
W(xu)гõԇ
1ԇ ɷ֣0.1g/ml NaOHҺ0.05g/ml CuSO4Һ÷ԇҺҺweϣٌϺԇy(c)ҺˮԡӟֱӼӟy(c)Һд߀ԭtʴutɫ
2Ƕԇ{(ln)ɫҺǻϺˮԡ(hu)F(xin)utɫǵĜy(c)
3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷y(c)Һȼ2mlҺuм3~4Һuy(c)дڵ|(zh)tʬF(xin)ɫ
4K÷ȡKw95%ľƾuڙzy(c)֬Ɍ֬ȾSɫKȾɼtɫ
5bDNADNAˮ(hu)Ⱦ{(ln)ɫ
6GbDNADNAGأ(hu)Ⱦ{(ln)Gɫ
750%ľƾҺ֬bУKȾҺȾɫ50%ľƾҺϴȥɫ
875%ľƾҺښ75%ľƾܝB뼚(x)(ni)ʹ|(zh)׃@(g)ƾĝBÓˮÜp(qing)@(g)t(hu)ʹ(x)浰|(zh)ѸÓˮ̳ĤKƾ75ľƾҺg(sh)ǰQˎᘾǰƤwÓԼC(j)е
995%ľƾҺswe?jn)?sh)95%ľƾDNA
1015%}95%ľƾҺweϿڽx
11đҺ(Ȟ0.01g/ml0.02g/ml)ȾɫwɫɌȾɫwȾɫͨȾɫ3~5犡ҲôtȾɫ
1220%ĸK3%^3.5%ȻFڱ^^øFe3+ĴЧʡrĸKк^ø
133%ĿԵҺ3%Һ2%røҺ̽ø(du)ۺǵÌ(sh)(yn)
14Һb۵Ĵ׃{(ln)
15ͪȡ~Gwеɫء
16Һɷ֣20ʯѡ2ݱͪ1ݱ϶Ҳ93̖(ho)ͣɫصČɫڞVϷx_
17裺ɫصȡķx(sh)(yn)ĥGɫ~Ƭr(sh)ʹĥ
18̼}ĥGɫ~Ƭr(sh)룬кЙC(j)ֹĥr(sh)~GwеɫƉ
190.3g/mLҺஔ(dng)30%Һֲ:(x)Һĝȴ|(zh)ڷx(sh)(yn)
200.1g/mLęcҺcuѪϣѪ
21ȻcҺٿܽDNA(dng)ȻcȞ2mol/L 0.015mol/Lr(sh)DNAܽȻcȞ0.14 mol/Lr(sh)DNAܽڝȞ0.9%r(sh)}ˮ
22ȵøٿÁֽ⵰|(zh)ڿڄ(dng):(x)B(yng)r(sh)ֽMʹM(x)ɢ
23ˮأ˹T(do)wԇȰl(f)ķNӻ磬ʹȾɫwMӱԭǿڷѵļ(x)Nwγ
24Ȼ}Ӽ(x)(x)ڵͨԣڻ̵D(zhun)ʹ(x)̎ڸܑB(ti)
֪R(sh)c(din)Y(ji)СP(gun)£
αһ֪R(sh)c(din)Y(ji)02-19
Y(ji)03-16
Z֪R(sh)c(din)֮ZR06-20
֪R(sh)c֪R(sh)c(din)07-28
ˮGij֪R(sh)c(din)10-19
tlj(mng)֪R(sh)c(din)12-20